|
|
| Nomenclature |
|
Symbol:
|
Mecp2tm2Bird
|
|
Name:
|
methyl CpG binding protein 2;
targeted mutation 2, Adrian Bird
|
|
MGI ID: |
MGI:3700191 |
|
Synonyms: |
Mecp2lox-stop, Mecp2stop |
|
Gene:
|
Mecp2
Location:
ChrX:74026592-74085690 bp, - strand
Genetic Position: ChrX,
37.63 cM
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:118365
|
|
Parent Cell Line:
| E14TG2a (ES Cell) |
|
Strain of Origin:
|
129P2/OlaHsd
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (Floxed/Frt) |
|
Mutation: | |
Insertion |
| |
|
Mutation details: Cre recombinase-reversible gene inactivation was accomplished by insertion into intron 2 of a 3.1-kb DNA fragment containing a loxP-flanked "neostop" cassette, which comprises a neomycin resistance cassette followed by a transcriptional/translational "stop" cassette composed of 550 bp of 3' sequence from the Saccharomyces cerevisiae His3 gene, an SV40 polyadenylation sequence, and a synthetic sequence containing a false translation initiation codon (ATG) immediately followed by a consensus splice donor sequence. Despite the production of a read-through transcript, no protein product was detected by western blot or immunofluorescence analysis of brains of mutant mice. (J:75205, J:111323, J:118365)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
| References |
|
Original: |
J:118365
Guy J et al.,
"Reversal of neurological defects in a mouse model of Rett syndrome."
Science 2007 Feb 23;315(5815):1143-7
|
|
All: |
10 reference(s)
|
|
Analysis Tools
