|
|
| Nomenclature |
|
Symbol:
|
Tg(PSEN1H163R)G9Btla
|
|
Name:
|
transgene insertion G9, Bruce Lamb
|
|
MGI ID: |
MGI:3693364 |
|
Synonyms: |
H163R mutant PS-1 YAC, H163R mutant PS-1 YAC transgenic mouse (line G9), (H163R) PS-1 YAC, PS-1 YAC transgenic line G9 |
|
Transgene:
|
Tg(PSEN1H163R)G9Btla
Location:
unknown
|
|
Transgene
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:58050
|
|
Parent Cell Line:
| J1 (ES Cell) |
|
Strain of Origin:
|
129S4/SvJae
|
|
Transgene
description |
|
Transgene
Type: | |
Transgenic (random, expressed) |
|
Mutation: | |
Insertion |
| |
|
Mutation details: A 1000 kb YAC transgene (788H12) containing the entire human presenilin-1 gene was altered to include the Familial Alzheimer's Disease- (FAD-) associated histidine-to-arginine substitution at amino acid position 163 (H163R). The transgene also includes approximately 550 kb of upstream and 350 kb of downstream flanking DNA. Semi-quantitative RT-PCR of multiple tissues shows expression of H163R mutant human mRNA at 50-70% of the level of wild-type mouse transcripts. Transgenic tissues express a wide spectrum of human presenilin-1 mRNA and protein species; alternatively spliced human mRNAs encoding presenilin 1, with or without the tetrapeptide VRSQ, are detected, and western blot analysis reveals accumulation of an 18-kDa C-terminal peptide. (J:58050)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
|
Notes |
The transgene construct was injected into embryonic stem (ES) cells.
|
| References |
|
Original: |
J:58050
Lamb BA et al.,
"Amyloid production and deposition in mutant amyloid precursor protein and presenilin-1 yeast artificial chromosome transgenic mice."
Nat Neurosci 1999 Aug;2(8):695-7
|
|
All: |
1 reference(s)
|
|
Analysis Tools
