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| Nomenclature |
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Symbol:
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Apobtm1Mae
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Name:
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apolipoprotein B;
targeted mutation 1, Nobuyo Maeda
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MGI ID: |
MGI:3665449 |
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Synonyms: |
apo b81 |
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Gene:
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Apob
Location:
Chr12:7977648-8016835 bp, + strand
Genetic Position: Chr12,
3.53 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:36426
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Parent Cell Line:
| E14TG2a (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: Using an 'in step' recombination strategy, the sequence encoding the putative binding domains in exon 26 were replaced with those encoding human HBB peptides. The 5' binding domain (3143LSVKAQYKKNSD3154) was replaced with amino acids 1-12 of the HBBS globin (1VHLTPVEKSAVT12). The 3' binding domain (3356GTSRLMRKRGL3366) was replaced with amino acids 120-131 of HBBA globin (120KEFTPPVQAAYQ131). The reading frame was maintained in the mutant allele, however, the disruption creates a premature stop codon at position 3688. The mutant allele contains 3687 residues, which is 81% of the full length protein.
(J:36426)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:36426
Toth LR et al.,
"Two distinct apolipoprotein B alleles in mice generated by a single 'in-out' targeting."
Gene 1996 31;178(1-2):161-8
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All: |
3 reference(s)
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Analysis Tools
