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| Nomenclature |
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Symbol:
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Tg(YACW408A5)1952Ricc
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Name:
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transgene insertion 1952, Andrea Riccio
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MGI ID: |
MGI:3655816 |
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Synonyms: |
YAC W408A5 |
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Transgene:
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Tg(YACW408A5)1952Ricc
Location:
unknown
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Transgene
origin |
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: An 800kb yeast artificial chromosome (YAC) designated W408A5, which contained C57BL/6 distal chromosome 7 sequence from the Cars to Th gene, and including imprinting center(IC2 which contains Cdkn1c (P57) and Kcnq1) and is syntenic to human chromosome 11p15.5, was modified by addition of 2 copies of the Neo-selection cassette. The construct was introduced into 129/Sv ES cells by yeast spheroplast fusion.
After maternal transmission of the transgene, most maternally expressed genes of the IC2 region (Phdla2, Slc22a18, Cdkn1c and Kcnq1) show 30-60% increased expression in the placenta relative to wild-type and equivalent or slightly reduced expression after paternal transmission. With paternal transmission, paternally expressed gene Kcnq1ot1 shows 2-fold increased expression over wild-type or after maternal transmission. Ascl2 expression (maternally expressed) is increased 2-fold after maternal or paternal transmission, while Tssc4 expression is increased 2-fold after maternal and by ~33% after paternal transmission. Imprinting of these 2 genes is not reproduced by the transgene. Expression of Cdkn1c and Kcnq1ot1 in the embryo is comparable to the placental expression (J:66461, J:96366)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:96366
Cerrato F et al.,
"The two-domain hypothesis in Beckwith-Wiedemann syndrome: autonomous imprinting of the telomeric domain of the distal chromosome 7 cluster."
Hum Mol Genet 2005 Feb 15;14(4):503-11
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All: |
2 reference(s)
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Analysis Tools
