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| Nomenclature |
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Symbol:
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Tg(Zp3-EGFP)1Dean
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Name:
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transgene insertion 1, Jurrien Dean
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MGI ID: |
MGI:3624514 |
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Synonyms: |
EGFP-MoZP3, EGFP-ZP3, EGFP-ZP3 (normal furin site), normal ZP3-EGFP |
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Transgene:
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Tg(Zp3-EGFP)1Dean
Location:
unknown
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Transgene
origin |
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Transgene
description |
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Transgene
Type: | |
Transgenic (Reporter) |
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Mutation: | |
Insertion |
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Mutation details: The ~8.1 kb transgene comprises the mouse Zp3 cDNA into which the the enhanced green fluorescent protein (EGFP) cDNA has been inserted downstream of the sequence encoding the 40 amino acid N-terminal signal peptide. Expression of the fusion gene is driven by ~6 kb of the oocyte-specific mouse Zp3 promoter. Ovulated ova and growing oocytes from transgenic mice exhibit EGFP fluorescence throughout the matrix of the zona pellucida; surrounding somatic cells do not fluoresce. Mature oocytes exhibit the strongest signal. Smaller, growing oocytes contain large, circular structures whose boundaries fluoresce and costain with BODIPY-Texas red ceramide.
(J:78932, J:86870)
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:78932
Zhao M et al.,
"Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice."
Mol Cell Biol 2002 May;22(9):3111-20
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All: |
3 reference(s)
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Analysis Tools
