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| Nomenclature |
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Symbol:
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Braftm1Cpri
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Name:
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Braf transforming gene;
targeted mutation 1, Catrin Pritchard
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MGI ID: |
MGI:3612533 |
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Synonyms: |
LSL-B-rafV600E, LSL-BRAFV600E |
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Gene:
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Braf
Location:
Chr6:39603237-39725463 bp, - strand
Genetic Position: Chr6,
18.43 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:104375
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Parent Cell Line:
| E14.1 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (Floxed/Frt) |
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Mutations: | |
Insertion, Single point mutation |
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Mutation details: A targeting vector was designed to insert a T1799A mutation into exon 15 and a loxP-stop-loxP cassette containing a minigene encoding exons 15-18 of wild-type Braf with a splice acceptor sequence at the 5' end into intron 14. Upon cre expression, the loxP-stop-loxP is deleted, enabling expression of the knock-in sequence. This mutation increases the kinase activity of the protein. (J:104375)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Braf Mutation:
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20 strains or lines available |
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Notes |
Phenotypic Similarity to Human Syndrome: Melanoma (tamoxifen-treated Braftm1Cpri/Braftm1Cpri Tg(Tyr-cre/ERT2)1Laru mice J:147434)
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| References |
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Original: |
J:104375
Mercer K et al.,
"Expression of endogenous oncogenic V600EB-raf induces proliferation and developmental defects in mice and transformation of primary fibroblasts."
Cancer Res 2005 Dec 15;65(24):11493-500
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All: |
7 reference(s)
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Analysis Tools
