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| Nomenclature |
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Symbol:
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Tg(Lim2*G15V)1Rlc
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Name:
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transgene insertion 1, Robert L Church
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MGI ID: |
MGI:3606782 |
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Synonyms: |
Lim2To3 transgene |
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Transgene:
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Tg(Lim2*G15V)1Rlc
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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(C57BL/6 x SJL)F2
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: The transgene comprises 6.3 kb of genomic DNA containing the entire mouse Lim2 gene, including 399 bp of 5' flanking DNA, with the G-to-T transversion in exon 2 putatively responsible for the dominant total opacity 3 (To3) phenotype. Dot-blot and Southern blot analyses of genomic DNA from transgenic mice indicate presence of two co-integrated copies. RT-PCR of total mouse lens RNA followed by digestion of the PCR product with BstE II, which cuts within the mutant, but not the wildtype, Lim2 cDNA, revealed a low but reproducible ratio of transgenic to wildtype mRNA in lenses from fetal (E18), weanling (P~20) and adult (P>20) transgenic mice.
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Inheritance: | |
Semidominant |
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Notes |
Only one founder, 26011F, transmitted the transgene and the eye phenotype; a second transgenic mouse, also having the eye phenotype, failed to transmit either. Absolute concordance of transgene dosage and the presence and severity of the eye phenotype in >100 progeny of founder 26011F and her offspring verified that the Lim2 mutation identified in To3 mice is responsible for the dominantly inherited To3 phenotype.
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| References |
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Original: |
J:100078
Steele EC Jr et al.,
"Lim2(To3) transgenic mice establish a causative relationship between the mutation identified in the lim2 gene and cataractogenesis in the To3 mouse mutant."
Mol Vis 2000 Jun 2;6():85-94
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All: |
1 reference(s)
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Analysis Tools
