|
|
| Nomenclature |
|
Symbol:
|
Tg(H2-Ea-Ins2)1Wehi
|
|
Name:
|
transgene insertion 1, William and Eliza Hall Institute
|
|
MGI ID: |
MGI:3590216 |
|
Transgene:
|
Tg(H2-Ea-Ins2)1Wehi
Location:
unknown
|
|
Transgene
origin |
|
Strain of Origin:
|
NOD/ShiLt
|
|
Transgene
description |
|
Transgene
Type: | |
Transgenic (random, expressed) |
|
Mutation: | |
Insertion |
| |
|
Mutation details: The transgene comprises nucleotides -1,903 through -39 of the 5' flanking sequence of the MHC Class II gene H2-Ea (k allele), containing the promoter, followed by a 909 base pair mouse (NOD strain) genomic DNA fragment containing the sequence encoding prepronsulin II and the SV40 small t intron and polyadenylation signal. Transgene-derived mRNA is detectable in spleen and thymus of transgenic NOD/LtWehi mice, but not of control littermates, by reverse transcription followed by PCR (RT/PCR) using either primers for H2-Eak and proinsulin or two proinsulin primers. Immunoreactive proinsulin is detectable in medium from cultured transgenic splenocytes only after 8 days' culture and at levels 10-2 - 10-3 times those of cultured islets or islet cells; levels are increased by culture with interferon gamma and tumor necrosis factor alpha. (J:100232)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
|
Notes |
Two lines of mice were created with this trasgenic construct on the NOD background. No distinction was made between them.
|
| References |
|
Original: |
J:100232
French MB et al.,
"Transgenic expression of mouse proinsulin II prevents diabetes in nonobese diabetic mice."
Diabetes 1997 Jan;46(1):34-9
|
|
All: |
4 reference(s)
|
|
Analysis Tools
