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| Nomenclature |
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Symbol:
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Atg7tm1Tchi
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Name:
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autophagy related 7;
targeted mutation 1, Tomaki Chiba
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MGI ID: |
MGI:3587769 |
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Synonyms: |
Atg7F, ATG7flox |
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Gene:
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Atg7
Location:
Chr6:114643097-114860595 bp, + strand
Genetic Position: Chr6,
53.05 cM, cytoband E3
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:100199
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Parent Cell Line:
| TT2 (ES Cell) |
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Strain of Origin:
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(C57BL/6NCrlj x CBA/JNCrlj)F1
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Mutation
description |
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Allele
Type: | |
Targeted (Floxed/Frt) |
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Mutation: | |
Insertion |
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Mutation details: A targeting construct was designed to insert a loxP site in intron 13. Exon 14 was fused to a cDNA encoding exons 15, 16, and 17 together with a polyadenylation signal after the stop codon. This was then followed by a neomycin resistance cassette and a second loxP site. An additional exon 14 sequence with a stop codon preceding the active site cysteine codon was inserted after the second loxP site. Immunoblot showed that the presence of the loxP sites and neo did not interefere with translation. (J:100199)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:100199
Komatsu M et al.,
"Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice."
J Cell Biol 2005 May 9;169(3):425-34
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All: |
45 reference(s)
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Analysis Tools
