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| Nomenclature |
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Symbol:
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Tg(Amelx)42Msnd
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Name:
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transgene insertion 42, Malcolm L Snead
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MGI ID: |
MGI:3584497 |
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Synonyms: |
M180deltaA-FLAG |
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Transgene:
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Tg(Amelx)42Msnd
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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(C57BL/6J x DBA/2J)F1 x CBA/J
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: The transgene consists of 2.3 kb of genomic DNA containing the amelogenin promoter region and the untranslated first exon, approximately 1.3 kb of intron 1, and the coding sequence for amelogenin - including the signal peptide necessary for secretion of the protein into the extracellular enamel space - with a 27-amino acid sequence containing the FLAG epitope replacing amino acids 3-42. The amelogenin stop codon is followed by the luciferase gene with its polyadenylation signal. (J:100004)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
In situ hybridization with a luciferase cDNA probe detects transgene mRNA only in ameloblasts. Immunohistochemical analysis of four day old incisors using antibodies to the FLAG epitope finds tagged amelogenin restricted to ameloblasts, where it is localized to the cytoplasm, and in the secreted organic enamel matrix. (J:100004)
Several independent founder lines generated from this construct were established; no differences were noted among their phenotypes, and no distinction was made among them.
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| References |
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Original: |
J:100004
Paine ML et al.,
"Enamel biomineralization defects result from alterations to amelogenin self-assembly."
J Struct Biol 2000 Dec;132(3):191-200
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All: |
6 reference(s)
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Analysis Tools
