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| Nomenclature |
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Symbol:
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Sema5atm1Awp
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Name:
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sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A;
targeted mutation 1, Andreas W Puschel
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MGI ID: |
MGI:3577050 |
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Synonyms: |
Sema5a- |
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Gene:
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Sema5a
Location:
Chr15:32244813-32696341 bp, + strand
Genetic Position: Chr15,
13.02 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:97663
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Parent Cell Line:
| R1 (ES Cell) |
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Strain of Origin:
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(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: A targeting vector was designed to replace exons 4 and 5 with a PGK-neo cassette. The null allele was achieved by inserting a stop codon after amino acid 121, thereby rendering a truncated protein unlikely to be biological active. Alternatively, aberrant splicing between exons 3 and 6 would result in the generation of a stop codon after amino acid 141. Western blot analysis of membrane fractions demonstrated a complete lack of protein in mutants. (J:97663)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Sema5a Mutation:
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5 strains or lines available |
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| References |
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Original: |
J:97663
Fiore R et al.,
"Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system."
Mol Cell Biol 2005 Mar;25(6):2310-9
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All: |
1 reference(s)
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Analysis Tools
