Tg(MMTV-rtTA)1Lach
Transgene Detail
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| Nomenclature |
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Symbol:
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Tg(MMTV-rtTA)1Lach
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Name:
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transgene insertion 1, Lewis A Chodosh
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MGI ID: |
MGI:3511875 |
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Synonyms: |
MMTV-rtTA, MMTV-rtTA-pA, MTB, Tg(rtTa)1Lach |
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Transgene:
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Tg(MMTV-rtTA)1Lach
Location:
unknown
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Transgene
origin |
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: The transgene comprises a 2.9-kb DNA fragment containing the MMTV LTR, 1.2 kb of LTR-upstream sequence, and 0.6 kb of v-Hras leader sequence; a 1.0-kb fragment containing a minigene encoding full-length reverse tetracycline transactivator (rtTA: "Tet-On"); and a 1.8 kb fragment containing the splicing and polyadenylation signals from SV40. Northern blot analysis readily detected rtTA transcripts in total mammary gland RNA from 5 week-old, doxycycline-treated, female transgenic mice. Upon induction by doxycycline, this transgene promoted high-level, homogeneous, reversible expression of beta-galactosidase or luciferase reporter transgenes in mammary epithelium, including terminal end buds, of female mice of various ages and reproductive states; expression was higher in pregnant and lactating mice than in virgin or post-involutional females. Except in older females continuously induced for at least 10 weeks, mammary glands of these mice were morphologically and functionally normal. rtTA and/or reporter expression was observed at lower levels in salivary gland, seminal vesicle and thymus, again only upon doxycycline administration. Older (15 weeks) induced bitrangenic female mice expressed beta-galactosidase in only 15-20% of mammary epithelial cells; however, levels of rtTA mRNA were only slightly lower than in 5 week-old induced mice. (J:75048)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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| References |
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Original: |
J:75048
Gunther EJ et al.,
"A novel doxycycline-inducible system for the transgenic analysis of mammary gland biology."
FASEB J 2002 Mar;16(3):283-92
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All: |
53 reference(s)
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Analysis Tools
