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| Nomenclature |
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Symbol:
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Tg(MMTVtTA)1Mam
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Name:
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transgene insertion 1, Lothar Hennighausen
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MGI ID: |
MGI:3053958 |
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Synonyms: |
MMTV-tTA |
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Transgene:
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Tg(MMTVtTA)1Mam
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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C57BL/6 and SJL
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: The MMTV LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin. The tetracycline resistance gene (TetR) was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). (J:92586)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
Additional lines were generated and showed comparable tissue expression patterns.
When transgenic mice were mated to mice transgenic for either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous patterns of reporter expression were observed in mammary epithelial cells and basal cells of the epidermis. Lower levels of reporter gene expression were also observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundredfold and was abrogated after the administration of tetracycline.
In combination with Tg(BCR/ABL1)2Dgt, when tetracycline administration is stopped, bitransgenic mice are models for B cell acute lymphoblastic leukemia (ALL). (J:72377)
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| References |
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Original: |
J:92586
Hennighausen L et al.,
"Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system."
J Cell Biochem 1995 Dec;59(4):463-72
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All: |
16 reference(s)
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