|
|
| Nomenclature |
|
Symbol:
|
Ghrhtm1Salv
|
|
Name:
|
growth hormone releasing hormone;
targeted mutation 1, Roberto Salvatori
|
|
MGI ID: |
MGI:3051561 |
|
Synonyms: |
GHRH-, GHRHKO |
|
Gene:
|
Ghrh
Location:
Chr2:157329497-157347506 bp, - strand
Genetic Position: Chr2,
78.25 cM
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:91828
|
|
Parent Cell Line:
| Not Specified (ES Cell) |
|
Strain of Origin:
|
129/Sv
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (knock-out) |
|
Mutations: | |
Insertion, Intragenic deletion |
| |
|
Mutation details: Part of intron 2, the last 3 amino acids of the N-terminal signal peptide and the first 14 amino acids of the mature protein, in exon 3, were replaced by a neomycin resistance gene. Southern blotting confirmed recombination in ES cells and in homozygous mice. RT-PCR indicated lack of transcript in mutant hypothalamus, testis and placenta. mRNA content in mutant pituitary was significantly less than that in wild-types and heterozygotes and Western blot confirmed this by showing a reduction in the intensity of protein in mutant pituitary and no protein in testes. (J:91828)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
| References |
|
Original: |
J:91828
Alba M et al.,
"A mouse with targeted ablation of the growth hormone-releasing hormone gene: a new model of isolated growth hormone deficiency."
Endocrinology 2004 Sep;145(9):4134-43
|
|
All: |
4 reference(s)
|
|
Analysis Tools
