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| Nomenclature |
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Symbol:
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Gabra6tm2(cre)Wwis
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Name:
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gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6;
targeted mutation 2, W Wisden
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MGI ID: |
MGI:3047798 |
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Synonyms: |
delta alpha6Cre |
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Gene:
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Gabra6
Location:
Chr11:42306437-42321072 bp, - strand
Genetic Position: Chr11,
25.03 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:91624
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Parent Cell Line:
| E14.1 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: Exon 8 was disrupted by insertion, via homologous recombination, of a TAG3 IRES-nlsCre-neo cassette, which contains stop codons in all reading frames followed by an encephalomyelocarditis virus internal ribosome entry site (IRES), the coding sequence for Cre recombinase flanked by the nuclear localization sequence and polyadenylation signal from SV40, and a neomycin resistance gene driven by the HSV promoter. Cre was shown, both by immunohistochemistry and by X-gal staining of brains of adult F1 progeny from a cross to a beta-galactosidase expressing Cre reporter strain, to be expressed uniformly in nuclei of all cells of the granule cell layer and, additionally, in a few Purkinje cells and in some small cells in the molecular layer. (J:91624)
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Recombinase activity
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:91624
Aller MI et al.,
"Cerebellar granule cell Cre recombinase expression."
Genesis 2003 Jun;36(2):97-103
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All: |
5 reference(s)
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Analysis Tools
