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| Nomenclature |
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Symbol:
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Tg(TcraTcrb)425Cbn
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Name:
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transgene insertion 425, Frank Carbone
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MGI ID: |
MGI:3046083 |
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Synonyms: |
OT2, OT-2, OTII, OT-II, OT-II.2, OT-II alphabeta TCR, OTII TCR, TCR-OT-II.2a, TCRTg, TgN(OT-II.2a), TgN(TcrOT-II), Tg(TcraTcrb)425-2Cbn |
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Transgene:
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Tg(TcraTcrb)425Cbn
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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C57BL/6 x B6.C-H2bm1
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: The transgene was generated by co-injection of two constructs created by separate cloning into a T-cell receptor expression vector the PCR-amplified cDNAs encoding mouse alpha-chain and beta-chain T-cell receptor subunits expressed by a CD4+ T-cell hybridoma designated Clone 1.1. The reconstituted receptor pairs with the CD4 coreceptor and is specific for chicken ovalbumin residues 323-339 in the context of H2-Ab1 (I-Ab). (J:87876)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
Transgenic mice express the mouse alpha-chain and beta-chain T-cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous transgenic mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T-cell ratio, and lymph node T-cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. Phenotypes for the two sublines (OT-II.2)425-2 and 426-6 are described as identical. (J:87876)
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| References |
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Original: |
J:87876
Barnden MJ et al.,
"Defective TCR expression in transgenic mice constructed using cDNA-based alpha- and beta-chain genes under the control of heterologous regulatory elements."
Immunol Cell Biol 1998 Feb;76(1):34-40
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All: |
694 reference(s)
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Analysis Tools
