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| Nomenclature |
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Symbol:
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Ebf2tm1Reed
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Name:
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early B cell factor 2;
targeted mutation 1, Randall R Reed
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MGI ID: |
MGI:3037977 |
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Synonyms: |
OE3/EBF2-tanGFP, O/E3GFP |
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Gene:
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Ebf2
Location:
Chr14:67233292-67430918 bp, + strand
Genetic Position: Chr14,
34.63 cM, cytoband D-E1
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:88585
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Parent Cell Line:
| R1 (ES Cell) |
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Strain of Origin:
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(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Mutation
description |
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Allele
Type: | |
Targeted (Reporter) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: A cassette containing tau-GFPpA reporter with a floxed tk-neo component was inserted in the place of exons 1-5 by homologous recombination, and remained under transcriptional control of the endogenous promoter. The exons encoded the first 162 amino acids, which contain domains crucial for DNA binding and transactivation of the protein binding site. Subsequent cre-mediated recombination removed the tk-neo cassette. In situ hybridization indicated lack of endogenous gene expression. The expression pattern of GFP recapitulated that of the endogenous gene. (J:88585)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Ebf2 Mutation:
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2 strains or lines available |
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| References |
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Original: |
J:88585
Wang SS et al.,
"Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection."
Development 2004 Mar;131(6):1377-88
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All: |
3 reference(s)
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Analysis Tools
