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| Nomenclature |
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Symbol:
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Cx3cr1tm1Litt
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Name:
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chemokine (C-X3-C) receptor 1;
targeted mutation 1, Dan R Littman
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MGI ID: |
MGI:2670351 |
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Synonyms: |
CX3CR1-, CX3CR1EGFP, CX3CR1-EGFP, Cx3cr1gfp, CX3CR1GFP, CX3CR1-GFP, CX3CR-GFP |
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Gene:
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Cx3cr1
Location:
Chr9:120048693-120068282 bp, - strand
Genetic Position: Chr9,
71.37 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:84544
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Parent Cell Line:
| E14.1 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (Reporter) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: The endogenous locus was disrupted by the insertion of sequence encoding green fluourescent protein (GFP), replacing the first 390 bp of the coding exon (exon 2). The deleted region encoded an amino-terminal portion of the protein that is crucial for interaction with endogenous ligand, Cx3cl1. A floxed neo gene included in the targeting vector for selection was excised prior to germline transmission, leaving a single loxP site downstream of the GFP sequence. RT-PCR and flow cytometry indicated an absence of endogenous protein and the presence GFP expression in homozygous mutant mice. (J:84544)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:84544
Jung S et al.,
"Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion."
Mol Cell Biol 2000 Jun;20(11):4106-14
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All: |
155 reference(s)
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Analysis Tools
