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| Nomenclature |
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Symbol:
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Krt14tm2Der
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Name:
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keratin 14;
targeted mutation 2, Dennis R Roop
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MGI ID: |
MGI:2653130 |
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Synonyms: |
mtK14loxP |
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Gene:
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Krt14
Location:
Chr11:100203162-100207548 bp, - strand
Genetic Position: Chr11,
63.43 cM, cytoband D
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Blistering on the left front leg and abdomen of Krt14tm2Der/Krt14+ pups
Show the 2 image(s) involving this allele.
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:67320
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Parent Cell Line:
| Not Specified (ES Cell) |
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Strain of Origin:
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129X1/SvJ
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: A C to T point mutation at codon 131 was introduced to the gene and a floxed PGK-neo cassette was inserted into the downstream exon via homologous recombination. The point mutation results in an arginine to cysteine substitution. Transient expression of cre recombinase in correctly targeted ES cells deleted the floxed PGK-neo cassette leaving a single loxP site in its place. The targeted allele was confirmed by Southern blot and sequence analysis. (J:67320)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Krt14 Mutation:
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20 strains or lines available |
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| References |
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Original: |
J:67320
Cao T et al.,
"An Inducible Mouse Model for Epidermolysis Bullosa Simplex. Implications for gene therapy."
J Cell Biol 2001 Feb 5;152(3):651-6
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All: |
1 reference(s)
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Analysis Tools
