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| Nomenclature |
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Symbol:
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Brca2tm1Kamc
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Name:
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breast cancer 2;
targeted mutation 1, Kimberly A McAllister
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MGI ID: |
MGI:2448890 |
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Synonyms: |
Brca2delta27 |
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Gene:
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Brca2
Location:
Chr5:150522630-150569746 bp, + strand
Genetic Position: Chr5,
89.52 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:75026
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Parent Cell Line:
| BK4 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: LoxP sites flanking exon 27 and a PGK-neo cassette were introduced via homologous recombination. Exon 27 was deleted in correctly targeted ES cells by transient expression of cre-recombinase. This exon encodes the C-terminal domain, which interacts with Rad51, contains a nuclear localization signal, and is required for maintenence of genomic stability in the event of DNA damage. RT-PCR analysis of testes RNA from mutant animals using exon 27-specific primers did not detect transcripts. However, authors were unable to confirm absence of protein expression because antibodies specific to this protein are currently not available. (J:75026)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:75026
McAllister KA et al.,
"Cancer susceptibility of mice with a homozygous deletion in the COOH-terminal domain of the Brca2 gene."
Cancer Res 2002 Feb 15;62(4):990-4
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All: |
6 reference(s)
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Analysis Tools
