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| Nomenclature |
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Symbol:
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Krt6a/Krt6btm1Cou
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Name:
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keratin 6B;
targeted mutation 1, Pierre A Coulombe
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MGI ID: |
MGI:2385808 |
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Synonyms: |
K6alpha/beta null |
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Gene:
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Krt6b
Location:
Chr15:101676034-101680289 bp, - strand
Genetic Position: Chr15,
56.97 cM
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Growth retardation and tongue blisters in Krt6a/Krt6btm1Cou/Krt6a/Krt6btm1Cou mice
Show the 3 image(s) involving this allele.
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:64033
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Parent Cell Line:
| CK35 (ES Cell) |
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Strain of Origin:
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129S2/SvPas
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intergenic deletion |
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Mutation details: Two closely linked genes were deleted using a single targeting event. The entire Krt2-6a gene, 13 kb of intergenic sequences, and the first half of Krt2-6b exon 1 were replaced with a neomycin resistance cassette via homologous recombination. RT-PCR and Northern blot analysis of cultured keratinocytes derived from homozygous mutant animals did not detect Krt2-6a or Krt2-6b transcripts, however a probe to the neo gene yielded positive results. Western blot analysis also failed to detect either Krt2-6a or Krt2-6b protein products. (J:64033)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Krt6b Mutation:
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0 strains or lines available |
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| References |
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Original: |
J:64033
Wong P et al.,
"Introducing a null mutation in the mouse K6alpha and K6beta genes reveals their essential structural role in the oral mucosa."
J Cell Biol 2000 Aug 21;150(4):921-8
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All: |
2 reference(s)
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Analysis Tools
