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| Nomenclature |
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Symbol:
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Gfaptm4Ito
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Name:
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glial fibrillary acidic protein;
targeted mutation 4, Shigeyoshi Itohara
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MGI ID: |
MGI:2384721 |
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Synonyms: |
Gfaphm3 |
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Gene:
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Gfap
Location:
Chr11:102887336-102900912 bp, - strand
Genetic Position: Chr11,
66.48 cM, cytoband D
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:78341
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Parent Cell Line:
| E14 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutations: | |
Insertion, Nucleotide substitutions, Intragenic deletion |
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Mutation details: The 5' portion of exon 1 was replaced with corresponding human sequence mutagenized to contain substitution mutations at all 5 phosphorylation sites, Thr7, Ser8, Ser13, Ser17, and Ser38 (human positions). The incorporation of the specific mutations was verified by sequence analysis as well as by Western blot analysis using antibodies directed toward the phosphorylation sites. Northern blot analysis of total brain RNA showed that transcript levels were unaffected in mutant mice. Immunohistochemical analysis of brain tissue showed that while the distribution of protein was conserved in mutant mice, protein levels were reduced in various regions. (J:78341)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:78341
Takemura M et al.,
"Protective role of phosphorylation in turnover of glial fibrillary acidic protein in mice."
J Neurosci 2002 Aug 15;22(16):6972-9
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All: |
1 reference(s)
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Analysis Tools
