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| Nomenclature |
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Symbol:
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Ntrk2tm1Lfr
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Name:
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neurotrophic tyrosine kinase, receptor, type 2;
targeted mutation 1, Louis F Reichardt
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MGI ID: |
MGI:2384391 |
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Synonyms: |
fBZ, trkBfbz, TrkBlox |
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Gene:
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Ntrk2
Location:
Chr13:58806596-59129976 bp, + strand
Genetic Position: Chr13,
31.2 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:69123
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Parent Cell Line:
| JM-1 (ES Cell) |
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Strain of Origin:
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129X1/SvJ
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Mutation
description |
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Allele
Type: | |
Targeted (Floxed/Frt) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: The first exon (exon S) was replaced with a floxed full-length cDNA copy of the gene followed by a PGK-neo-loxP cassette and a tau-lacZ cassette. The PGK-neo-loxP cassette was removed in correctly targeted ES cells via transient expression of cre recombinase, leaving the floxed cDNA and tau-lacZ cassette intact. Expression of the floxed cDNA is under the control of the endogenous promoter. The tau-lacZ sequence is not transcribed due to SV40 polyadenylation signals and multiple stop codons following the floxed cDNA. Northern blot analysis of brain from homozygous mutant animals shows the presence of only full-length transcripts. Multiple alternative transcripts observed in wild-type animals are absent. Gene expression and protein expression in mutant animals is approximately 33% and 24% that of wild-type, respectively. Immunohistochemical analysis demonstrated that only full-length protein, and no protein isoforms, are expressed in mutant animals, and the pattern of expression is similar to wild-type. (J:69123)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:69123
Xu B et al.,
"The role of brain-derived neurotrophic factor receptors in the mature hippocampus: modulation of long-term potentiation through a presynaptic mechanism involving TrkB."
J Neurosci 2000 Sep 15;20(18):6888-97
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All: |
15 reference(s)
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Analysis Tools
