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| Nomenclature |
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Symbol:
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Nrp2tm1.2Mom
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Name:
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neuropilin 2;
targeted mutation 1.2, Peter Mombaerts
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MGI ID: |
MGI:2183904 |
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Synonyms: |
Neuropilin-2-Delta, NP2-delta, Nrp2del, Nrp2tm1Mom |
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Gene:
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Nrp2
Location:
Chr1:62703285-62818695 bp, + strand
Genetic Position: Chr1,
31.35 cM, cytoband C2
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:76686
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Parent Cell Line:
| E14 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (Reporter) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: A loxP site 272 bp upstream of the translation initiation codon was introduced into exon 1 and a loxP-GFP FRT-flanked neo cassette was inserted 1 kb into intron 1 via homologous recombination. Heterozygous animals were crossed to an flp recombinase transgenic line for removal of the neo cassette. Subsequent mating to a cre recombinase transgenic line removed exon 1 and part of intron 1 leaving the GFP cassette intact. The exon 1 deletion removes the plasma membrane-targeting signal sequence and also brings the GFP cassette under the control of the endogenous promoter. RT-PCR analysis confirmed the removal of the signal sequence in gene transcripts from homozygous mutant animals. Immunohistochemistry of brain sections from homozygous mutants did not detect protein product. (J:76686)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:76686
Walz A et al.,
"Aberrant sensory innervation of the olfactory bulb in neuropilin-2 mutant mice."
J Neurosci 2002 May 15;22(10):4025-35
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All: |
7 reference(s)
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Analysis Tools
