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| Nomenclature |
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Symbol:
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Ptprctm1Mak
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Name:
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protein tyrosine phosphatase, receptor type, C;
targeted mutation 1, Tak W Mak
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MGI ID: |
MGI:2181288 |
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Synonyms: |
cd45-, CD45-exon6- |
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Gene:
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Ptprc
Location:
Chr1:138062861-138175305 bp, - strand
Genetic Position: Chr1,
60.73 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:13163
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Parent Cell Line:
| Other (see notes) (ES Cell) |
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Strain of Origin:
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Not Specified
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: Exon 6, which is excised in some isoforms, was deleted and replaced with a neomycin selection cassette. Immunofluorescence analysis, using an antibody raised against all isoforms, of cells isolated from homozygous mutant mice showed an absence of encoded protein in B lymphocytes and low levels of expression in peripheral T cells. Flow cytometric analysis of peripheral T cells confirmed that protein containing the region encoded by exon 6 was absent, while protein containing the region encoded by exon 5 was present at low levels. (J:13163)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Ptprc Mutation:
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49 strains or lines available |
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Notes |
ES cell line = D3NS.
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| References |
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Original: |
J:13163
Kishihara K et al.,
"Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice."
Cell 1993 Jul 16;74(1):143-56
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All: |
37 reference(s)
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Analysis Tools
