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| Nomenclature |
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Symbol:
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Itgb4tm1Fgg
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Name:
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integrin beta 4;
targeted mutation 1, Filippo G Giancotti
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MGI ID: |
MGI:2181069 |
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Synonyms: |
Beta4TL |
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Gene:
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Itgb4
Location:
Chr11:115974709-116008412 bp, + strand
Genetic Position: Chr11,
80.91 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:48924
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Parent Cell Line:
| CJ7 (ES Cell) |
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Strain of Origin:
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129S1/Sv-Oca2+ Tyr+ Kitl+
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutation: | |
Insertion |
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Mutation details: A cassette containing a stop codon, a SV40 polyadenylation site, and a neomycin resistance gene was inserted immediately downstream of the exon encoding the transmembrane domain (upstream of sequence encoding the cytoplasmic domain). Expression analyses showed the presence of truncated transcript and protein in mutant mice. While Northern blot analysis detected increased levels of mutant transcript, Western blot analysis showed protein levels to be comprable to wild-type. FACS analysis confirmed that mutant protein was correctly expressed on the cell surface. (J:48924)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Itgb4 Mutation:
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5 strains or lines available |
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| References |
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Original: |
J:48924
Murgia C et al.,
"Cell cycle and adhesion defects in mice carrying a targeted deletion of the integrin beta4 cytoplasmic domain."
EMBO J 1998 Jul 15;17(14):3940-51
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All: |
1 reference(s)
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Analysis Tools
