|
|
| Nomenclature |
|
Symbol:
|
Tgm2tm1Gml
|
|
Name:
|
transglutaminase 2, C polypeptide;
targeted mutation 1, Gerry Melino
|
|
MGI ID: |
MGI:2180638 |
|
Synonyms: |
Tg2-, TG2-, TG2KO, Tgase2- |
|
Gene:
|
Tgm2
Location:
Chr2:158116402-158146436 bp, - strand
Genetic Position: Chr2,
78.72 cM
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:67573
|
|
Parent Cell Line:
| Not Specified (ES Cell) |
|
Strain of Origin:
|
Not Specified
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (knock-out) |
|
Mutations: | |
Insertion, Intragenic deletion |
| |
|
Mutation details: A 1.2 kb region of the gene spanning part of exon 5, intron 5, exon 6, and part of exon 6 was replaced with a neo resistance cassette via homologous recombination thus deleting the active site of exon 6 and results in the loss of protein cross-linking activity. Western blot of liver, thymus, brain, and erythrocyte samples and RT-PCR analysis of thymus from homozygous mutant animals verified the absence of gene expression. However, residual enzyme activity was detected in some tissues which the authors speculate may be attributed to Tgase1. (J:67573)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
| References |
|
Original: |
J:67573
De Laurenzi V et al.,
"Gene disruption of tissue transglutaminase."
Mol Cell Biol 2001 Jan;21(1):148-55
|
|
All: |
28 reference(s)
|
|
Analysis Tools
