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| Nomenclature |
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Symbol:
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Cd86tm2Shr
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Name:
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CD86 antigen;
targeted mutation 2, Arlene H Sharpe
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MGI ID: |
MGI:2159105 |
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Synonyms: |
B7-1/2-, B7-1/B7-2-, B7.1/B7.2-, B7DKO, B7KO, Cd80/86- |
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Gene:
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Cd86
Location:
Chr16:36603869-36666081 bp, - strand
Genetic Position: Chr16,
25.72 cM, cytoband B5
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:39089
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Parent Cell Line:
| J1 (ES Cell) |
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Strain of Origin:
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129S4/SvJae
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: To create a knockout of both Cd80 and Cd86 in mice, an ES cell line in which the Cd80 gene was mutated was subsequently used in a second round of gene targeting. A hygromycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences of the Cd86 gene. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele. (J:39089)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:39089
Borriello F et al.,
"B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation."
Immunity 1997 Mar;6(3):303-13
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All: |
90 reference(s)
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Analysis Tools
