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| Nomenclature |
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Symbol:
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Gnai2tm1Lbi
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Name:
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guanine nucleotide binding protein (G protein), alpha inhibiting 2;
targeted mutation 1, Lutz Birnbaumer
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MGI ID: |
MGI:2152603 |
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Synonyms: |
alphai2-, Gai2-, Galphai2-, Galphai2-KO, Gnai2-, Gnai2tm1Uru |
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Gene:
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Gnai2
Location:
Chr9:107614138-107635342 bp, - strand
Genetic Position: Chr9,
58.43 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:194592
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Parent Cell Line:
| AB1 (ES Cell) |
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Strain of Origin:
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129S7/SvEvBrd-Hprt+
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutation: | |
Insertion |
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Mutation details: A neomycin resistance cassette was inserted into exon 3. The truncated protein is predicted to be inactive and also lack the cysteine at position 352 which is ADP-ribosylated by pertussis toxin. No protein was detected in homogenates from homozygous homozygous mice by ADP-ribosylation with pertussis toxin. The absence of protein was also confirmed by immunoblotting. (J:24434, J:194592)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Gnai2 Mutation:
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37 strains or lines available |
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| References |
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Original: |
J:194592
Rudolph U et al.,
"Disruption of the G(i2) alpha locus in embryonic stem cells and mice: a modified hit and run strategy with detection by a PCR dependent on gap repair."
Transgenic Res 1993 Nov;2(6):345-55
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All: |
56 reference(s)
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Analysis Tools
