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| Nomenclature |
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Symbol:
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Ppargtm1Rev
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Name:
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peroxisome proliferator activated receptor gamma;
targeted mutation 1, Ronald M Evans
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MGI ID: |
MGI:2149838 |
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Synonyms: |
Pparg-, PPARg- |
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Gene:
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Pparg
Location:
Chr6:115361221-115490401 bp, + strand
Genetic Position: Chr6,
53.41 cM, cytoband E3-F1
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:58223
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Parent Cell Line:
| J1 (ES Cell) |
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Strain of Origin:
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129S4/SvJae
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Mutation
description |
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Allele
Type: | |
Targeted (Reporter) |
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Mutation: | |
Insertion |
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Mutation details: In-frame insertion of a lac Z-neomycin resistance cassette into the second coding exon of the gene, just upstream of the DNA binding domain, eliminated both DNA-binding and ligand-binding functions of the receptor. Whole-mount beta-galactosidase assays revealed conspicuous beta-gal activivity in the interscapular brown fat pad of only E14.5 embryos carrying a targeted allele, but not in wild-type embryos (J:58223)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
Complementation of Ppargtm1Rev mutant embryos with wild-type placenta by tetraploid aggregation corrects the cardiac defect and extends viability to E12.5 (two mutants out of a total five), E15.5 (one out of ten) and at birth (one of six). Rescued embryos survive to term but exhibit a second lethal phase during the first week of life. Additional phenotypes include: a 30% reduction in body weight (P0-P4) and subsequent ill health (as shown by dehydration, weight loss and lethargy starting at P5) associated with lipodystrophy, a severly pale, distended and fatty liver, severe intestinal bleeding, and numerous focal hematomas throughout the brain (J:58223).
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| References |
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Original: |
J:58223
Barak Y et al.,
"PPAR gamma is required for placental, cardiac, and adipose tissue development."
Mol Cell 1999 Oct;4(4):585-95
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All: |
12 reference(s)
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Analysis Tools
