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| Nomenclature |
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Symbol:
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Apoetm1Bres
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Name:
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apolipoprotein E;
targeted mutation 1, Jan L Breslow
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MGI ID: |
MGI:2137814 |
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Synonyms: |
apo E-, apoE-, EKO, epsilon- |
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Gene:
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Apoe
Location:
Chr7:19696109-19699166 bp, - strand
Genetic Position: Chr7,
9.94 cM
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Gross appearance of aortic arches of Apoetm1Bres/Apoetm1Bres, Plgtm1Jld/Plgtm1Jld and Apoetm1Bres/Apoetm1Bres Plgtm1Jld/Plgtm1Jld mice
Show the 3 image(s) involving this allele.
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:2809
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Parent Cell Line:
| E14 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: A neomycin resistance cassette replaced 1.4kb of the gene, including part of the promoter through part of the second intron. Northern blot analysis using a mouse exon 4 riboprobe did not detect wild-type message in livers from homozygous mutant mice, but did detect a longer transcript that the authors propose is a neo-Apoe fusion product. Gradient SDS-PAGE analysis did not detect full size Apoe associated with any fractions from plasma lipoproteins from homozygous mutant mice. (J:2809)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
In Apoetm1Bres mutant mice also carrying Csf1op, atherotic lesions of the proximal aorta were abolished or significantly reduced in size relative to Apoe mutants homozygous for the normal allele at Csf1 (J:29024).
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| References |
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Original: |
J:2809
Plump AS et al.,
"Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells."
Cell 1992 Oct 16;71(2):343-53
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All: |
108 reference(s)
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Analysis Tools
