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| Nomenclature |
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Symbol:
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Krastm2Tyj
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Name:
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v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog;
targeted mutation 2, Tyler Jacks
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MGI ID: |
MGI:2135928 |
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Synonyms: |
K-ras2LA1, K-rasLA1, KrasLA1 |
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Gene:
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Kras
Location:
Chr6:145216699-145250239 bp, - strand
Genetic Position: Chr6,
77.37 cM, cytoband G2
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:68981
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Parent Cell Line:
| D3 (ES Cell) |
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Strain of Origin:
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129S2/SvPas
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutations: | |
Insertion, Nucleotide substitutions |
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Mutation details: Insertion of the targeting vector results in a duplication of exon 1 with a neomycin resistance gene between the duplicate exons. The upstream (inserted) exon 1 contains an activating glycine to aspartic acid mutation at codon 12. In the absence of intrachromosomal recombination, this allele does not produce any protein and mutant homozygotes die during embryogenesis. In heterozygotes, when intrachromosomal recombination occurs in vivo, half of the recombination events produce an active mutant allele coding for the G12D amino acid substitution and half produce a wild-type allele. Expression of G12D protein results in the formation of lung tumors and death at ~300 days (10 months). (J:68981)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:68981
Johnson L et al.,
"Somatic activation of the K-ras oncogene causes early onset lung cancer in mice."
Nature 2001 Apr 26;410(6832):1111-6
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All: |
15 reference(s)
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Analysis Tools
