|
|
| Nomenclature |
|
Symbol:
|
Parp1tm1Hsmm
|
|
Name:
|
poly (ADP-ribose) polymerase family, member 1;
targeted mutation 1, Hiroshi Suzuki Mitsuko Masutani
|
|
MGI ID: |
MGI:1857765 |
|
Synonyms: |
Parp- |
|
Gene:
|
Parp1
Location:
Chr1:180568975-180601254 bp, + strand
Genetic Position: Chr1,
84.44 cM, cytoband H5
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:53360
|
|
Parent Cell Line:
| J1 (ES Cell) |
|
Strain of Origin:
|
129S4/SvJae
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (knock-out) |
|
Mutation: | |
Insertion |
| |
|
Mutation details: A neomycin resistance cassette was inserted into exon 1, 10 bp downstream of the ATG codon. Protein activity was not detected in tissues derived from homozygous mice. Western blot analysis detected a 113 kDa protein in homozygous mice, but this truncated form is predicted to be inactive beacuse it should lack a critical N-terminal zinc finger. (J:53360)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
|
Notes |
Null mice appear healthy and fertile with no gross abnormalities. No skin hyperplasia or obesity was observed. These mice were used to examine the role of ADPRP in Streptozotocin induced diabetes. STZ injection caused a significant increase in blood glucose concentrations of wildtype mice as well as necrosis in islet cells leading to an eventual loss on beta cells. In contrast, homozygous mice are protected from STZ-induced diabetes. Glucose levels in these animals remained relatively normal and islet beta cell death was markedly less in these animals (J:53360).
|
| References |
|
Original: |
J:53360
Masutani M et al.,
"Poly(ADP-ribose) polymerase gene disruption conferred mice resistant to streptozotocin-induced diabetes."
Proc Natl Acad Sci U S A 1999 Mar 2;96(5):2301-4
|
|
All: |
12 reference(s)
|
|
Analysis Tools
