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| Nomenclature |
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Symbol:
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Pomctm1Low
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Name:
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pro-opiomelanocortin-alpha;
targeted mutation 1, Malcolm J Low
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MGI ID: |
MGI:1857481 |
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Synonyms: |
betaend-, beta-END-, End-, POMCX*4- |
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Gene:
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Pomc
Location:
Chr12:3954951-3960618 bp, + strand
Genetic Position: Chr12,
1.99 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:47711
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Parent Cell Line:
| D3 (ES Cell) |
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Strain of Origin:
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129S2/SvPas
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutation: | |
Insertion |
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Mutation details: A PGK-neomycin based targeting vector was used to introduce a premature start codon into exon 3, resulting in the truncation of the translated protein. In situ hybridization on homozygous mutant animals demonstrates that the truncation event had no effect on the distribution or level of transcript. Immunoreactivity demonstrated that Homozygous mutant mice had no detectable beta endorphin activity in the hypothalamus or pituitary (J:78759)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
Additional reference J:65685 identifies the ES cell line origin as 129S2/SvEv. It is more likely that the mice used in this additional reference were derived from the targeted D3 ES cells (129S2/SvPas) first generated in J:78759 and implanted in mice in J:47711.
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| References |
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Original: |
J:47711
Rubinstein M et al.,
"Absence of opioid stress-induced analgesia in mice lacking beta-endorphin by site-directed mutagenesis."
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):3995-4000
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All: |
29 reference(s)
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Analysis Tools
