|
|
| Nomenclature |
|
Symbol:
|
Alpltm1Sor
|
|
Name:
|
alkaline phosphatase, liver/bone/kidney;
targeted mutation 1, Philippe Soriano
|
|
MGI ID: |
MGI:1857124 |
|
Synonyms: |
Tnap-, TN-APBetageo, TNAP-bgeo |
|
Gene:
|
Alpl
Location:
Chr4:137741733-137796384 bp, - strand
Genetic Position: Chr4,
70.02 cM
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:25249
|
|
Parent Cell Line:
| AB1 (ES Cell) |
|
Strain of Origin:
|
129S7/SvEvBrd-Hprt+
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (knock-out) |
|
Mutations: | |
Insertion, Intragenic deletion |
| |
|
Mutation details: A neomycin cassette replaced 1.6 kb of the gene, from a portion of exon 2 to a portion of exon 6. The construct deleted the active site of the protein, Ser 93, located in exon 5. RT-PCR of total RNA from E13 gonads of homozygous mutant mice did not detect transcript. In addition, lack of protein in homozygous mutant mice was demonstrated functionally. (J:25249)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
|
Notes |
Only the embryonic (EAP) and tissue non-specific (TNAP) forms of alkaline phosphatase are expressed in mouse embryos. EAP is the predominant form of the enzyme in the early embryo, but predominance switches to TNAP about the 7th embryonic day. Akp2tm1Sor was used to clarify the expression of TNAP in mouse primordial germ cells. These cells do not express TNAP before gastrulation, but the enzyme is elaborated by extraembryonic tissue prior to that stage. Primordial germ cells do not require TNAP for either development or migration (J:25249).
|
| References |
|
Original: |
J:25249
MacGregor GR et al.,
"Tissue non-specific alkaline phosphatase is expressed in both embryonic and extraembryonic lineages during mouse embryogenesis but is not required for migration of primordial germ cells."
Development 1995 May;121(5):1487-96
|
|
All: |
8 reference(s)
|
|
Analysis Tools
