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| Caption | Spermatogenic defects in Katnal2tm1a(EUCOMM)Wtsi/Katnal2tm1a(EUCOMM)Wtsi Tg(Stra8-icre)1Reb/0 mice. (a) Testis weight in Katnal2tm1c(EUCOMM)Wtsi/Katnal2tm1c(EUCOMM)Wtsi (Katnal2FLOX/FLOX) (white triangles) and Katnal2tm1c(EUCOMM)Wtsi/Katnal2tm1c(EUCOMM)Wtsi Tg(Stra8-icre)1Reb/0 (Katnal2GCKO/GCKO) (black triangles) mice (n = 3/group). (b) Daily sperm output (DSP) in the testes of FLOX/FLOX (white triangles; n = 3) and GCKO/GCKO (black triangles; n = 4) mice. (c) Total epididymal sperm content of FLOX/FLOX (white triangles) and GCKO/GCKO (black triangles) mice (n = 3/group). Total epididymal sperm content was reduced by 94.1% in GCKO/GCKO mice compared to FLOX/FLOX mice. In a-c lines represent mean SD, ** p<0.01 compared to FLOX/FLOX. Periodic acid Schiff's (PAS) stained testis sections from FLOX/FLOX and GCKO/GCKO mice (d-i and p-q). Low magnification view of seminiferous tubules in FLOX/FLOX (d) and GCKO/GCKO (e) mice. Multinucleated symplasts (arrowhead) were frequently observed in the GCKO/GCKO seminiferous epithelium. Elongating spermatids in FLOX/FLOX (f) versus GCKO/GCKO (g) mice. Abnormal nuclear morphology of spermatids (arrowheads) was frequently observed in GCKO/GCKO mice. Spermiation in FLOX/FLOX (h) versus GCKO/GCKO (i) mice. Retained elongated spermatids (arrowheads) were often observed in stage IX tubules of GCKO/GCKO mice but were rarely observed in FLOX/FLOX mice. Acetylated tubulin immunolabelling as a marker for sperm tail presence in FLOX/FLOX (j) and GCKO?GCKO (k) testis sections. Sperm tail content was markedly reduced in GCKO/GCKO (k) versus WT (j) mice. Scale bars in d-k = 10 um. Electron microscopy of stage VIII tubulobulbar complexes (asterisks) in FLOX/FLOX (l) versus GCKO/GCKO (m) mice. Tubulobulbar complex formation was normal in GCKO/GCKO mice. Scale bars in l-m= 2 um. Immunostaining of seminiferous tubules for the tubulobulbar complex marker ARP2 in FLOX/FLOX (n) versus GCKO/GCKO (o) mice further confirmed the presence of tubulobulbar complexes from GCKO/GCKO spermatids. Residual body (arrowheads) formation in FLOX/FLOX (p) versus GCKO/GCKO (q) mice. Residual body formation appeared normal in GCKO/GCKO mice. Scale bars in n-q = 10 um. | ||||||
| Copyright | This image is from Dunleavy JEM, PLoS Genet 2017 Nov;13(11):e1007078, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:250115 | ||||||
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/09/2024 MGI 6.23 |
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