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Caption Effect of genetic depletion of 11-cis-retinal production on retinal degeneration. A, whole retinal images obtained from postnatal day (PND) 36 Rhotm1.1Kpal/Rho+ (P23H/+) and Rhotm1.1Kpal/Rho+ Lrattm1Kpal/Lrattm1Kpal (P23H/+ Lrat-/-) littermate mice. Plastic sections were made from dorsal-ventral axis cut and stained with toluidine blue. Both mice were heterozygous with respect to the L450M variation of RPE65 (RPE65450L/450M). The outer nuclear layer (ONL), observed as a purple line in the P23H/+ mouse retina (left panel), was almost gone and only retained near the cilial marginal zone (CMZ) in the P23H/+ Lrat-/- mouse (right panel; indicated by curved line). B, high magnification retinal images shown in A and Rhotm1.1Kpal/Rho+ Lrattm1Kpal/Lrattm1Kpal mice at PND 28-30. Images were taken from the midpoint between optic nerve (ON) and CMZ. Genetic deletion of 11-cis-retinal production promoted retinal degeneration in the P23H/+ mouse at PND 36 but not in Rho+/- Lrat-/- mouse at PND 28-30. In the P23H/+ Lrat-/- mouse, however, the ROS were almost undetectable, and the number of photoreceptor nuclei was dramatically reduced compared with a littermate that had 11-cis-retinal (P23H/+).
Copyright This image is from Sakami S, J Biol Chem 2011 Mar 25;286(12):10551-67 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:170648
Symbol Name
Lrattm1Kpal lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase); targeted mutation 1, Krzysztof Palczewski
Rhotm1.1Kpal rhodopsin; targeted mutation 1.1, Krzysztof Palczewski
Rpe65450L retinal pigment epithelium 65; 450 Leucine
Rpe65450M retinal pigment epithelium 65; 450 Methionine
Allelic Composition Genetic Background
Rhotm1.1Kpal/Rho+ involves: 129S6/SvEvTac * C57BL/6 * FVB/N
involves: 129S6/SvEvTac * C57BL/6 * FVB/N

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