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Phenotype Image Detail
Image
Caption Conditional inactivation of Neurog3 in only the intestine results in a complete loss of all enteroendocrine cells all along the proximal-distal axis of the intestine. Sections of adult duodenum, jejunum, ileum, and colon were examined for the presence of endocrine cells in control and Neurog3tm1Fgu/Neurog3tm3Ggr Tg(Vil-cre)20Syr/0 or Neurog3tm3Ggr/Neurog3tm3Ggr Tg(Vil-cre)20Syr/0 (mutant) animals by immunofluorescence. Images presented are from the jejunum of control (A, C, E, G, and I) and mutant (B, D, F, H, and J) animals and are representative for the general loss of all enteroendocrine cells in mutant animals. Villin-Cre-mediated inactivation of Neurog3 results in a complete loss of all Neurog3+ enteroendocrine progenitors (B), which in wild-type (WT) animals are located in the intestinal crypt compartment (A, arrows). Likewise, mutant animals are also devoid of chromogranin A+ (D), Cck/gastrin+ (F), Glp1+ (H), and Gip+ (J) cells, normally located in the villi of WT mice (arrows in C, E, G, and I), respectively. The age of the animals analyzed is 10-12 weeks. Original magnification, 10.
Copyright This image is from Mellitzer G, J Clin Invest 2010 May 3;120(5):1708-21 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:161480
Associated
Alleles
Symbol Name
Neurog3tm1Fgu neurogenin 3; targeted mutation 1, Francois Guillemot
Neurog3tm3Ggr neurogenin 3; targeted mutation 3, Gerard Gradwohl
Tg(Vil-cre)20Syr transgene insertion 20, Sylvie Robine
Associated
Genotypes
Allelic Composition Genetic Background
Neurog3tm1Fgu/Neurog3tm3Ggr
Tg(Vil-cre)20Syr/0
involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL
Neurog3tm3Ggr/Neurog3tm3Ggr
Tg(Vil-cre)20Syr/0
involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL

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last database update
12/05/2017
MGI 6.11
The Jackson Laboratory