Phenotype Image Detail

Image

Caption

(C) PAS staining in the thalamus shows weak staining in the Hexb⁺/Hexb^tm1Rlp (Hexb+/- animal) (i) and strong staining in neurons of the Sandhoff animal (Hexb^tm1Rlp/Hexb^tm1Rlp Tg(Hexb-tTA2S,tetO-Hexb)#Tjsa/0 (Hexb-/-Hex^Tg) or Hexb^tm1Rlp/Hexb^tm1Rlp Tg(SYN1-tTA2S,tetO-Hexb)#Tjsa/0 (Hexb-/-SYN^Tg)) at humane endpoint (iv, magenta staining). Hexb^tm1Rlp/Hexb^tm1Rlp Tg(Hexb-tTA2S,tetO-Hexb)#Tjsa/0 and Hexb^tm1Rlp/Hexb^tm1Rlp Tg(SYN1-tTA2S,tetO-Hexb)#Tjsa/0 animals were protected from accumulation of lipids in the thalamus, shown by a lack of PAS staining (ii and iii). In animals that were fed doxycycline, PAS staining revealed significant accumulation of glycoconjugates (v and vi). Sections were counterstained with haematoxylin. Scale bar = 50 um.

This image is from Sargeant TJ, PLoS Genet 2012 Sep;8(9):e1002943, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:190450

Associated
Alleles

Symbol	Name
Hexb^tm1Rlp	hexosaminidase B; targeted mutation 1, Richard L Proia
Tg(Hexb-tTA2S,tetO-Hexb)#Tjsa	transgene insertion, Timothy J Sargeant
Tg(SYN1-tTA2S,tetO-Hexb)#Tjsa	transgene insertion, Timothy J Sargeant

Associated
Genotypes

Allelic Composition	Genetic Background
Hexb^tm1Rlp/Hexb^tm1Rlp Tg(Hexb-tTA2S,tetO-Hexb)#Tjsa/0	involves: 129S4/SvJae * C57BL/6 * CBA
Hexb^tm1Rlp/Hexb^tm1Rlp Tg(SYN1-tTA2S,tetO-Hexb)#Tjsa/0	involves: 129S4/SvJae * C57BL/6 * CBA

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