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| Caption | Hematoxylin and eosin staining (A and B), immunohistochemical analysis for expression of ADAR (ADAR1) by using an ADAR-specific mAb (C and D) and for TUNEL assays (E-H) were conducted with serial sections of wild-type (+/+) and Adartm1.1Knk/Adartm1.1Knk (-/-) embryos recovered live at E11.0. For immunostaining analysis of mutant embryo sections (D), the final color development step was carried out for twice as long (10 min) as in the control experiment (C) done for wild-type embryo sections so that at least the outline of the mutant embryos can be seen. Otherwise, immunostaining of mutant embryo sections was in practice blank. By using the same ADAR-specific mAb, Western blot analysis was carried out for protein samples extracted from whole embryo bodies (E11.0), demonstrating the absence of both normal and aberrant ADAR protein products in mutant embryos (right panel). TUNEL assays carried out on whole embryos (E and F) and liver sections (G and H) reveal widespread apoptosis in tissues stained dark silver gray. The highest levels of apoptosis were detected in heart (ht), liver (lv) and vetebra (vt). Examples of apoptotic hepatocytes (black arrowheads) and hematopoietic cells (red arrowheads) are indicated (H). | ||||
| Copyright | This image is from Wang Q, J Biol Chem 2004 Feb 6;279(6):4952-61 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:87714 | ||||
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Associated Alleles |
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/09/2024 MGI 6.23 |
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