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| Caption | Generation of the Cyp51tm1Bfro and Cyp51tm1.1Bfro alleles. A, targeting strategy. The neomycin resistance cassette (gray box) flanked with two FRT sites (encircled black triangle) was inserted into intron 3. Exons 3 and 4 (black boxes) were flanked by two loxP sites (squared white triangle) inserted into introns 2 and 4 (together with new HindIII restriction sites). The thymidine kinase (TK) gene (gray arrows) was used for negative selection of non-targeted insertion events. Diagnostic HindIII (H) and BamHI (B) restriction sites as well as probes for Southern analysis (black bars) located 5' (5'-out) and 3' (3'-out) to both homology arms are indicated. Gray arrows show PCR primer positions used for genotyping. Crossed bars indicate homologous recombination. The neomycin selection cassette was removed in ES cell clones with Flp recombinase generating a conditional Cyp51tm1Bfro allele. EIIa-Cre recombinase was used to obtain the knock-out Cyp51tm1.1Bfro allele lacking exons 3 and 4. | ||||||
| Copyright | This image is from Keber R, J Biol Chem 2011 Jun 25;(): and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:173366 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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