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| Caption | Generation of the Acantm1(cre/ERT2)Crm allele. Top panel displays the genomic structure of the mouse aggrecan gene. Middle panel shows the targeting vector. Bottom panel depicts the targeted allele whereby IRES-CreERT2 and an FRT-flanked Pgk-Neo cassette (in reverse transcriptional orientation to aggrecan) is recombined into the 3' untranslated region (3'UTR) of the aggrecan gene. EcoRV restriction enzyme digestion is used in Southern blotting analysis to identify correctly targeted ES cell cones. Both the 5' and 3' probes (position shown in the middle panel) detect a 13.5-kb fragment with the wild-type allele. The targeted allele generates a 5.4- or 9.6- kb fragment detected by the 5' or 3' probe, respectively. At the bottom of the Panel (a) targeted ES clone (3H11) is shown to the left in a Southern blot hybridized with the radiolabeled 5' probe. The Southern blot of the 3' probe with the same clone is shown to the right. RV: EcoRV sites. TK: MC1-TK. Neo: Pgk-Neo. FRT: FRT sites. | ||||
| Copyright | This image is from Henry SP, Genesis 2009 Dec;47(12):805-14, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:155982 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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