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| Caption | Generation of the Prkcitm2.1(Prkcz)Ltg allele. Targeting of the Prkci (PKCi) locus in mice. Schematic representation of the targeting strategy is shown. Restriction maps indicating the relevant restriction sites of the wild-type (wt) and mutant locus are shown (B, BamHI; Bg, BglII; E, EcoRI; EV, EcoRV; H, HindIII; K, Kpnl). The black box represents the second exon of the Prkci gene which was used for all targeted modifications. The white boxes below the mutant alleles indicate the 5' HindIII-EcoRI RFLP probe (5' probe) and the 3' KpnI-EcoRI RFLP probe (3' probe) used for later southern blot analysis. C) Rescue knockin (PKCifRes) allele - Prkci wild-type (wt) locus, the targeting construct (containing an inserted Prkcz cDNA into the coding sequence of the 2nd exon followed by a floxed neo cassette) and the mutant Prkci locus (PKCiota/lambda Res allele) after homologous recombination and subsequent deletion of the neo cassette via Cre recombinase are shown. | ||||
| Copyright | This image is from Seidl S, PLoS One 2013;8(5):e62756, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:202124 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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