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| Caption | Generation of Vps18 conditional knockout mice. (A) Schematic representation of the knockout strategy for Vps18 gene. (1) Genomic DNA fragment of Vps18 gene containing exon 2-5. (2) Schematic structure of the Vps18 targeting vector. (3) Genomic structure of Vps18tm1Wtao (Vps18galeo) allele after homologous recombination. (4) Genomic structure of Vps18tm1.1Wtao (Vps18F) allele after removal of nLacZ and pgk-Neo expression cassettes simultaneously by FLP. pgk-DTA and pgk-Neo represent the diphtheria toxin A and the neomycin expression cassettes respectively. nLacZ represents a modified E. coli beta-galactosidase gene containing nuclear localization signal at its N-terminal. The exons are numbered and depicted by open boxes. The open and black triangles denote loxP and FRT sequence respectively. The gray box before nLacZ represents a splicing acceptor. Xba I sites are indicated by short vertical lines. The gray ellipse in (3) depicts the probe for Southern blot analysis. | ||||||
| Copyright | This image is from Peng C, J Biol Chem 2012 Aug 1;(): and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:186307 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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