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Caption A, strategy for disrupting the mouse Kcne3 gene. Top model, wild-type (WT) allele with exons depicted as boxes. Exon 4 contains the entire coding region. Lower models, targeting construct containing a neomycin cassette (neo) as positive and the diphtheria toxinA cassette (dta) and thymidine kinase (tk) as negative selection markers. The neomycin cassette was flanked by loxP sites (black triangle). A third loxP site was inserted after exon 4. In the recombined allele, the neomycin cassette introduced a new BglI restriction site, giving rise to 6- and 8-kb BglI restriction fragments. This results in the floxed Kcne3tm1Tjj allele. The Kcne3tm1.1Tjj allele (bottom) is obtained by Cre-recombinase mediated excision of exon 4. A 9.5-kb BglI restriction fragment (that is shorter than the 12-kb fragment of the WT allele) may be detected with 5' and 3' probes (bars at bottom) by Southern blotting.
Copyright This image is from Preston P, J Biol Chem 2010 Mar 5;285(10):7165-75 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:160721
Symbol Name
Kcne3tm1.1Tjj potassium voltage-gated channel, Isk-related subfamily, gene 3; targeted mutation 1.1, Thomas J Jentsch
Kcne3tm1Tjj potassium voltage-gated channel, Isk-related subfamily, gene 3; targeted mutation 1, Thomas J Jentsch

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