Phenotype Image Detail

Image

Caption

Aberrant cardiomyocyte oranization and contractility in Hnrnpu^tm1.1Tman/Hnrnpu^tm1.1Tman Tg(Ckmm-cre)5Khn/0 (Ckmm-KO) hearts. (A) Mutant hearts are dilated and display disorganized cardiomyocytes compared with controls. Shown is the H&E staining of P14 heart sections of both control and mutant hearts. A low-magnification view of the heart and high-magnification views of cardiomyocyte arrangements are shown. Trichrome staining of P14 heart sections and WGA & DAPI staining for P11 heart sections are also shown. (Black scale bar, 40 um; white scale bar in Far Right panels, 10 um.) (B) Abnormal actin dynamics and shortened sarcomere in mutant hearts. Heart sections (P11) were stained with phalloidin and antibodies against Desmin to reveal actin fiber and sarcomere Z line, respectively. (Scale bar, 10 um.) (C) Mutant cardiomyocytes have contractility defects. Control and mutant hearts (P11) were perfused under relaxing conditions; heart sections were prepared and examined by electron microscopy. Two green lines spanning 8.772 um were drawn in both control and mutant samples to compare the unit length of sarcomeres.

Copyright

This image is from Ye J, Proc Natl Acad Sci U S A 2015 Jun 9;112(23):E3020-9. Copyright 2015 National Academy of Sciences, U.S.A. J:223139

Associated Alleles

Symbol	Name
Hnrnpu^tm1.1Tman	heterogeneous nuclear ribonucleoprotein U; targeted mutation 1.1, Tom Maniatis
Tg(Ckmm-cre)5Khn	transgene insertion 5, C Ronald Kahn

Associated Genotypes

Allelic Composition	Genetic Background
Hnrnpu^tm1.1Tman/Hnrnpu^tm1.1Tman Tg(Ckmm-cre)5Khn/0	involves: 129S4/SvJaeSor * FVB