Phenotype Image Detail

Image

Caption

Pancreatic neuroendocrine tumors (PNETs) and dysplastic endocrine cells in Gcgr^tm1Jcp/Gcgr^tm1Jcp mice at 10-12 months. (A-P) PNETs in mutant mice. (A) A PNET (arrow) inside the tail of a fixed whole pancreas from a 12-month mutant mouse. (B and C) H&E staining of the tumor in A (4x and 20x, respectively). (D) Immunostaining for chromogranin A (CGA, green) of the tumor in A. (E-L) PNETs from 10-12-month mutant mice were immunostained for glucagon (Glu) (E, green), somatostatin (SST) (F, green), insulin (Ins) (G and K, red), pancreatic polypeptide (PP) (H and L, green), Ki-67 (I, pink), and cyclin D1 (J, pink). (M) H&E staining of liver with metastatic PNET foci. (N) Focal fat replacement of exocrine pancreas with preservation of dysplastic islets. (O and P) A PNET co-expressing insulin (O, red) and glucagon (P, green). (Q-T) Dysplastic endocrine cells from 12-month mutant mice immunostained for islet hormones. (Q and R) Pancreas from mutant mice was double-stained for insulin (red) and glucagon (green). Orange or yellow denotes overlap of red and green fluorescence. (S and T) Pancreas from mutant mice was stained for pancreatic polypeptide (S) or somataostatin (T) (both green). Blue represents nuclei in fluorescent images. White scale bar, 250 um; red scale bar, 125 um. The scale bar in E applies to E-P except for I and J.

Copyright

This image is from Yu R, PLoS One 2011;6(8):e23397, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:176507

Associated Alleles

Symbol	Name
Gcgr^tm1Jcp	glucagon receptor; targeted mutation 1, Janice C Parker

Associated Genotypes

Allelic Composition	Genetic Background
Gcgr^tm1Jcp/Gcgr^tm1Jcp	DBA/1LacJ-Gcgr^tm1Jcp