Phenotype Image Detail

Image

Caption

Aberrant proliferation in Rassf9^{Tg(PDLIM7*)2Cml}/Rassf9^{Tg(PDLIM7*)2Cml} (-/-) skin. (A) BrdU incorporation (green) and K14 (red) were co-stained by immunofluorescence in the skin cross-sections of two-week-old mutant (^-/-; right panel) and wild-type (WT) mice (+/+; left panel). Areas marked by yellow dashed line are enlarged in the adjacent column ("Enlarge"). Scale bar = 100 um. ORS, outer root sheath; HB, hair bulb; Epi, epidermis; Der, dermis; Adi, adipose. (B) High-magnification images of BrdU incorporation in dermal tissues. Abnormal patterns of proliferating cells in Epi, ORS and HB of ^-/- skin are consistent with those observed in (A). The dashed white lines denote the epidermis-dermis border. Similar results were obtained from three independent pairs of mice. Scale bar = 20 um. (C) Skin proliferation was evaluated by quantitation of BrdU incorporation using ImageJ software. Analyses were performed for the number of BrdU-positive nuclei in basal layer (top panel; per mm of epidermis); the number of BrdU-positive nuclei in dermal hair follicles (middle panel; per mm² of dermis layer); and the percentage of HB with more than five BrdU-positive nuclei (bottom panel). Data represent scoring of at least 20 hair bulbs from each mouse and three mice for each genotype. *, P<0.05; **, P<0.001.

Copyright

This image is from Lee CM, PLoS One 2011;6(3):e17867, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:171677

Associated Alleles

Symbol	Name
Rassf9^Tg(PDLIM7*)2Cml	Ras association (RalGDS/AF-6) domain family (N-terminal) member 9; transgene insertion 2, Chiou-Mei Lee

Associated Genotypes

Allelic Composition	Genetic Background
Rassf9^Tg(PDLIM7*)2Cml/Rassf9^Tg(PDLIM7*)2Cml	involves: ICR