Phenotype Image Detail

Image

Caption

Smad4 deficiency significantly enhanced MT-TGFalpha-induced epithelial expansion. Increased number of proliferating ductal cells was observed in the Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^{tm1.1(cre)Cvw}/Ptf1a⁺ Tg(MtTGFA)Lmb/0 (STP) mice over the Tg(MtTGFA)Lmb/0 (MT-TGFalpha) mice. (A) Ki-67 immunolabeling of pancreata from MT-TGFalpha (panel i) and STP (panel ii) mice after 8-months of zinc sulfate treatment. Arrows denote proliferating epithelial cells in evolving acinar-to-ductal metaplasia (ADM) and PanIN lesions. (B) The graph presents the percentage of Ki-67-positive epithelial cells among the total number of metaplastic cells counted. The table shows the percentage of Ki67-positve cells and in parentheses the total counts of Ki67-positive cells over the total number of metaplastic cells counted from at least three independent mice per genotype and ten 400x fields per mouse are shown. Values are presented as mean SE; Magnifications panels i and ii, 400x. (Student's t-test, * p < 0.05; ** p < 0.01).

Copyright

This image is from Garcia-Carracedo D, PLoS One 2015;10(3):e0120851, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:229184

Associated Alleles

Symbol	Name
Ptf1a^tm1.1(cre)Cvw	pancreas specific transcription factor, 1a; targeted mutation 1.1, Christopher VE Wright
Smad4^tm1.1Gsu	SMAD family member 4; targeted mutation 1.1, Gloria H Su
Tg(MtTGFA)Lmb	transgene insertion, Glenn Merlino

Associated Genotypes

Allelic Composition	Genetic Background
Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^tm1.1(cre)Cvw/Ptf1a^+ Tg(MtTGFA)Lmb/0	involves: 129S1/Sv * 129X1/SvJ * CD-1
Tg(MtTGFA)Lmb/0	involves: CD-1