Phenotype Image Detail

Image

Caption

Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^tm1Brn Tg(Pbsn-cre)4Prb/0 (Brca2^F/F;Trp53^F/F;PBCre4) prostate intraepithelial neoplasia (PIN) lesions proliferate post-castration. Control (Brca2^F/F;Trp53^F/F) and Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^tm1Brn Tg(Pbsn-cre)4Prb/0 (mutant) prostates were surgically castrated at 16 months and culled 4 days later. (A) Haematoxylin and eosin stain shows atypical cells are still present in mutant prostates 4 days after castration, but there is a reduction in cells present in the lumen. Right panels show detail of neoplasia, atypical cells are indicated with arrowheads. (B) Androgen receptor (AR) immunohistochemistry shows expression 4 days post-castration is predominantly throughout the cytoplasm of control and mutant prostates. Arrows indicate cells that have higher nuclear AR expression. Right panels show detail of cells with nuclear AR expression, indicated with arrowheads. (C) TUNEL assay analysis demonstrates an increase in apoptotic cells in control and mutant prostates after castration. Arrows indicate some apoptotic cells. (D) Ki-67 immunohistochemistry shows there are proliferating cells in castrated mutants. LP is lateral prostate, AP is anterior prostate.

Copyright

This image is from Francis JC, PLoS Genet 2010;6(6):e1000995, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:161511

Associated Alleles

Symbol	Name
Brca2^tm1Brn	breast cancer 2, early onset; targeted mutation 1, Anton Berns
Tg(Pbsn-cre)4Prb	transgene insertion 4, Pradip Roy-Burman
Trp53^tm1Brn	transformation related protein 53; targeted mutation 1, Anton Berns

Associated Genotypes

Allelic Composition	Genetic Background
Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^tm1Brn Tg(Pbsn-cre)4Prb/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2